Therapeutic drug monitoring by point-of-care analysis:
The Guiducci lab coordinates a national Nano-Tera project (ISyPeM) which involves the Department of Clinical Pharmacology at CHUV, since decades at the forefront of scientific research and clinics approaches in therapeutic drug monitoring. The project consortium is committed to develop a stand-alone system for drug analysis and support for dose adjustment in patients under long-term treatments in the framework of the widespread of an individualized medicine approach. The Guiducci lab developed a guided capture-SELEX protocol for DNA aptamers addressed to the minimization of the number of selection cycles and to the selection against the drug and the metabolites. The DNA aptamer for tobramycin is specific and could detect and quantify the drug concentration in serum samples by direct label-free detection.
Multi tri-gate nanodevices for ultra-scaled pH measurement
Recently presented bioanalytical integrated systems based on polymerase reaction-monitoring through pH measurements lean toward sensor arrays of everâ€‘increasing density by relying, up to a certain extent, on the scalability of CMOS microfabrication technology. The integration density is, however, limited when employing standard planar field-effect devices, due to the larger impact of noise on smaller devices. We demonstrated that the employment of top-down silicon nanowires in a multi-wire configuration counteracts the effect of noise by leveraging the enhanced conductivity properties and increased surface of nano-sized triâ€‘gate devices. We showed that the signal of silicon nanowires does not scale with size and that a highlyâ€‘relevant resolution of less than 0.0025 pH is attainable with sensors occupying a surface in the sub-micrometer square range.
Individually addressable sensing by silicon-based posts and 3D electrodes for lab-on-a-chip applications
Mastering vertical electrodes integrated in microfluidic chamber leads to well-controlled actuation and high SNR impedance biosensing. The Guiducci lab developed an innovative technology based on silicon, metals and SU8 to obtain vertical microelectrodes and posts integrated in tens of micron high channel. The resulting devices have superior performance in cell detection and manipulation.
Heterogeneous integration of arrays of biosensors on CMOS chip, enabled by innovative 3D integration solutions for chip-to-chip stacking and semi-disposable systems: The Guiducci lab in collaboration with LSM_EPFL introduced the concept and developed the technology of a disposable top layer of a 3D chip dedicated to biosensing and fluidic functions.